A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area
نویسندگان
چکیده
Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (DA) and the measured area (MA) for assessing fungicide growth inhibition of the ascomycete, Phyllosticta citricarpa on solid media. Both the calculated, DA and the actual measured area, MA were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however MA was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.
منابع مشابه
Splash dispersal of Phyllosticta citricarpa conidia from infected citrus fruit
Rain-splash dispersal of Phyllosticta citricarpa (syn. Guignardia citricarpa) conidia (pycnidiospores) from infected oranges was studied in still air and combined with wind. High power microscopy demonstrated the presence of conidia in splash droplets from diseased oranges, which exuded conidia for over one hour during repeated wetting. The largest (5 mm) incident drops produced the highest spl...
متن کاملFirst report of Phyllosticta citricarpa and description of two new species, P. paracapitalensis and P. paracitricarpa, from citrus in Europe
The genus Phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. Phyllosticta citricarpa is the causal agent of Citrus Black Spot disease (CBS), affecting fruits and leaves of several citrus hosts (Rutaceae), and can also be isolated from asymptomatic citrus tissues. Citrus Black Spot occurs in citrus-growing regions with warm summer rainfall cli...
متن کاملSusceptibility of Fruits of the ‘Valência’ and ‘Natal’ Sweet Orange Varieties to Guignardia citricarpa and the Influence of the Coexistence of Healthy and Symptomatic Fruits
The importance of the presence of symptomatic fruits with citrus black symptoms (CBS) on the disease severity level in subsequent crop production was assessed in an orchard planted with Valência and Natal sweet orange varieties. Additionally, the period of susceptibility of the fruits of these varieties was evaluated. Fruits were covered with paper bags at the stage of 75% fallen petals and wer...
متن کاملA morphologic and anatomic evaluation of furcation area in the upper and lower first molars
A morphologic and anatomic evaluation of furcation area in the upper and lower first molars Dr. V. Esfahanian* - Dr. M. Ketabi* - Dr. M. Hafezibakhtiari** - Dr. SH. Ashtari** *- Assistant Professor of Periodontics Dept. - Faculty of Dentistry – Isfahan Islamic Azad University (Khorasgan). **- Dentist. Background and Aim: Microbial plaque has been recognized as the main cause of periodontal dise...
متن کاملEndophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot
We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a ta...
متن کامل